03
Feb
In contrast, just a fraction of the cells in the tumor tissues demonstrated significant positive staining for CD90, which range from 1.5 to 15.1?%. or nontarget control (RiboBio, NC#22, China) had been co-transfected using the vectors for 48?hours, and luciferase activity was measured then. Clone and sphere development assay For the clone development assay, 500 cells had been sorted by MACS and seeded per well in 6-well plates. After 10?times of lifestyle, the clones were fixed using methanol and dyed with hematoxylin, and the amount of clones ( 50 cells) was assessed microscopically. For the sphere development assay, 1000 cells…