05
Apr
In this scenario, a presynaptic targeting signal might reside outside this region, between amino acids 1 and 52. generating an antibody against phosphorylated Mover and Western blot analysis of fractionated rat brain, we found that Mover is a phospho-protein. The localization of Mover to synaptic vesicles is phosphorylation dependent; treatment with a phosphatase caused Mover to dissociate from synaptic vesicles. A yeast-2-hybrid screen, co-immunoprecipitation and cell-based optical assays of homomerization revealed that Mover undergoes homophilic interaction, and regions within both the N- Acacetin and C- terminus of the protein are required for this interaction. Deleting a region required for homomeric…