The clinicopathological characteristics were extracted from the patients clinical charts, which included symptoms, radiological findings, laboratory data, and surgical procedures as well as the outcome. Results: There were 13 men and 27 women (sex-ratio M/F = 0,48) aged between 24 and 76 years (mean = 65 years). the SNOMED taxonomy were established. For remaining terms, we explored whether these could be defined by combining pre-existing (more simple) SNOMED CT concepts. This process (‘post-coordination) is well supported by SNOMED CT and allows extending its content. Results: Of glomerular terms, 88/195 (45%) could be matched to SNOMED CT. %-matching was more successful for KBC terms designating disease concepts (56%) than patterns of injury (32%). For the majority of terms that could not be mapped, we found that these could indeed be defined as a compositional expression of pre-existing SNOMED CT concepts (post-coordination). Sildenafil We suggested concepts that are needed for this post-coordination. Conclusion: SNOMED CT is considered the standard for documenting, encoding and exchanging medical data in/between health information systems. This proof-of-concept shows that mapping of KBC terms to SNOMED CT is feasible, in part directly, in part through post-coordination. OFP-01-002 Molecular analysis of renal transplant biopsies comparing the Edmonton Molecular Microscope with the NanoString Human Organ Transplant Panel J. Schmitz*, H. Stark, S. Bartels, D. Jonigk, P.F. Halloran, G. Einecke, J.H. Br?sen *Institute of Pathology, Nephropathology Sildenafil Unit, Hannover Medical School, Germany Background & objectives: Different molecular methods like microarrays or quantitative PCRs were used by several groups on renal transplant tissues. High-resolution determination of the inflammatory infiltrate by NanoString analysis (which was developed for formalin-fixed paraffin-embedded-derived RNA) should be a sufficient approach. Methods: We used surveillance and indication biopsies from 63 patients whose time-matched second biopsy core had been frozen and analysed by microarray in the INTERCOM/INTERCOMEX study. After re-evaluation according to recent Banff consensus, RNA isolation was performed with Maxwell FFPE kits and led Sildenafil to sufficient RNA yields in 53 samples which were further processed for NanoString analysis (Human Organ Transplant panel). Results: Morphologically, of the 53 samples analysed (samples from 2011/12 and 2015), twenty-five patients showed no signs of rejection, twelve had borderline rejection, four showed cellular rejection, seven had humoral rejection, and five presented with combined rejection. Preliminary analysis of gene expression by T-distributed Stochastic Neighbour Embedding (t-SNE), Random Forest and Principal Component Analysis (PCA) showed clear differences between samples with rejection (humoral and cellular) and without rejection. Rejection samples revealed high expression of chemokine ligands compared to rejection-free tissues. Borderline rejection shared a common pattern compared to samples without rejection. First results display good correlation with the former molecular diagnosis from the INTERCOM/INTERCOMEX study. Conclusion: Molecular approach using the NanoString platform may supplement morphological diagnosis of renal grafts especially in unclear cases and thus enhance precision diagnostics with small tissue requirement. Morphological and molecular evaluation in the same biopsy core from FFPE tissue enables direct histological-molecular correlation. Additionally, this technology also improves our understanding of pathophysiology in renal and other transplants. Funding by: Dr. Werner Jackst?dt foundation OFP-01-003 Arteriolar C4d: a potential prognostic marker in IgA nephropathy C a retrospective study in a Portuguese tertiary centre P. Amoroso Can?o*, B. Faria, Q. Cai, C. Henriques, A.C. Matos, F. Poppelaars, M. Gaya da Costa, M. R. Daha, R. Pestana Silva, M. Pestana, M. A. Seelen *Centro Hospitalar Universitrio de S?o Jo?o, Portugal Background & goals: IgA Nephropathy (IgAN) may be the most common glomerulonephritis worldwide. C4d continues to be named a marker connected with reduced renal success significantly. We aimed to review the clinical need for arteriolar immunoexpression of C4d within a cohort of IgAN sufferers. Strategies: We chosen all kidney biopsies using the medical diagnosis of IgAN, between 2001 Mouse monoclonal to CD21.transduction complex containing CD19, CD81and other molecules as regulator of complement activation and 2017, and analyzed their scientific features; examined them based on the Oxford Classification of IgAN 2016 and evaluated the current presence of vascular lesions. We examined the arteriolar and glomerular immunoexpressions of C4d and their association using the baseline and follow-up clinico-histological data believed bivariate and regression evaluation. Outcomes: Arteriolar immunoexpression of C4d was within 21 (17%) biopsies and connected with mean arterial pressure (MAP), persistent microangiopathy and arterial intimal fibroelastosis. After changing to various other significant predictors, such as for example baseline approximated glomerular filtration price, MAP and the current presence of crescents, this immunoexpression remained associated.
The clinicopathological characteristics were extracted from the patients clinical charts, which included symptoms, radiological findings, laboratory data, and surgical procedures as well as the outcome
