***p=0.0002, **p=0.0013, ns?=?not really significant (t check). Amount 2source data 1.An Excel sheet with numerical data over the quantification of indicated Jurkat cell lines analyzed for MT radiality on the immunological synapse and total 3D MT company of Jurkat cells during polarization represented as plots in Amount 2C,G and F.Click here to see.(12K, xlsx) Figure 2figure dietary supplement 1. Open in another window Characterization of microtubule?(MT) network in polarizing T cells by Extension Microscopy?(ExM).(A) PD0166285 Quantification of total estimated MT length per cell indicated in indicated Jurkat T cell lines. (8.8K) GUID:?2FB3D99F-F604-4022-8AB5-017D0A9BAEDB Amount 3source data 1: An Excel sheet with numerical data over the quantification of KIF21B-GFP velocities, single-molecule analysis of KIF21B-GFP in Jurkat cells and the consequences of one KIF21B-GFP substances on developing microtubule leads to Jurkat cells represented as frequency distributions (Amount 3B), frequency distributions with lognormal meets (Amount 3C), so that as plots (Amount 3G, H and We). elife-62876-fig3-data1.xlsx (587K) GUID:?DE031E3B-D06B-417D-A522-FFCBB828356F Amount 3figure dietary supplement 1source data 1: An Excel sheet with numerical data over the quantification of single-molecule analysis of KIF21B-GFP in Jurkat cells and the consequences of one KIF21B-GFP molecules in developing microtubule leads to Jurkat cells represented being a lognormal data in PD0166285 shape (Amount 3figure dietary supplement 1B) so that as plots (Amount 3figure dietary supplement 1B and F). elife-62876-fig3-figsupp1-data1.xlsx (1.7M) GUID:?08B1D959-62D2-432B-B976-34F3FE3697A0 Figure 4source data 1: An Excel sheet with numerical data over the quantification of indicated Jurkat cell lines Rabbit polyclonal to ABCA6 for MT dynamics parameters and centrosome polarization toward the immunological synapse represented as plots (Figure 4B, C, E and I) and cumulative frequency distributions (Figure 4F and H). elife-62876-fig4-data1.xlsx (54K) GUID:?92E1E6D2-6634-4974-9310-8BC81F004B94 Amount 4figure dietary supplement 1source data 1: An Excel sheet with numerical data over the quantification of indicated Jurkat cell lines for MT pause durations represented being a story in Amount 4figure dietary supplement 1A. elife-62876-fig4-figsupp1-data1.xlsx (13K) GUID:?DE30EBD3-9D6E-4D01-B434-892D830F7B3D Amount 5source data 1: A CSV document with numerical data of represented trajectories as plotted in Amount 5D, left -panel. The mean MT amount of the symbolized story is usually obtained from each trajectory for every 0.4 s of simulation. elife-62876-fig5-data1.zip (1.8M) GUID:?9EFDF286-48DE-4179-90FC-88D2FDC44EBB Physique 5source data 2: A CSV file with numerical data of mean MT length at time t?=?300 s as plotted in Determine 5D, right panel. Values were obtained from the trajectories represented in the left panel of Physique 5D. elife-62876-fig5-data2.zip (1.8K) GUID:?F2F42D0A-D4A9-42B0-AB4E-0ED5FB433C07 Figure 5source data 3: A CSV file with numerical data of represented trajectories as plotted in Figure 5F, left panel. The mean MT length of the represented plot is usually obtained from each trajectory for every 0.4 s of simulation. elife-62876-fig5-data3.zip (1.1M) GUID:?1F5399EF-6885-44D1-A5E4-1989AB7AA038 Figure 5source data 4: A CSV file with numerical data of mean MT length at time t?=?300 s as plotted in Determine 5F, right panel. Values were obtained from the trajectories represented in the left panel of Physique 5F. elife-62876-fig5-data4.zip (1.2K) GUID:?F7171F23-92DE-4EB0-A564-7A80FD747184 Physique 5figure product 1source data 1: A CSV file with numerical data of the fraction of MT surviving as a function of time. The analytical functions are indicated, and the experimental data is usually represented as a reverse cumulative distribution as plotted in Physique 5figure PD0166285 product 1A. elife-62876-fig5-figsupp1-data1.zip (671 bytes) GUID:?11104F1A-2CAC-48C9-AF94-F2BD348D5908 Figure 5figure product 1source data 2: A CSV file with numerical data of MT surviving under force as a function of catastrophe rates for different MT forces exerted by polymerization. This data is usually represented in Physique 5figure product 1C. elife-62876-fig5-figsupp1-data2.zip (3.6K) GUID:?F6BE27CC-8DB7-440B-B885-A9F2A332EE3E Physique 5figure supplement 1source data 3: A CSV file with numerical data of the free catastrophe rate as a function of the growing force, used to obtain a linear fit and represented in Physique 5figure supplement 1D. elife-62876-fig5-figsupp1-data3.zip (488 bytes) GUID:?35BC88BB-9640-4C4E-8DFD-E19B8DDA12E3 Physique 6source data 1: A CSV file with numerical data of polarization time as a function of catastrophe rates represented in Physique 6C. elife-62876-fig6-data1.zip (1.2K) GUID:?FF5D5D24-378C-4FA2-9FFE-C9E85E4458DC Physique 6source data 2: A CSV file with numerical data of polarization time as a function of the numbers of KIF21B motors represented PD0166285 in Physique 6D. elife-62876-fig6-data2.zip (880 bytes) GUID:?338BFB37-B53A-4A23-A604-B4A7429C2EAF Physique 6source data 3: A CSV file with numerical data of the distance from your centrosome to the synapse as a function of time PD0166285 for different numbers of KIF21B motors, shown in Physique 6E. elife-62876-fig6-data3.zip (562K) GUID:?29D2E792-17D3-4AE4-9F61-7DF19E7FF880 Physique 6source data 4: A CSV file with numerical data of pressure imbalance per time trace and per time point for different numbers of KIF21B motors, as plotted in Physique 6F. elife-62876-fig6-data4.zip (1.0M) GUID:?2980794D-A75C-4CE2-B76F-52DD1E6CBB73 Figure 6source data 5: A CSV file with numerical data of the mean numbers of MT bound to dynein for different numbers of KIF21B motors, as shown in Figure 6G. elife-62876-fig6-data5.zip (27K) GUID:?6DCA5643-9A6B-494C-8B00-405EE1DE8770 Figure 6source data 6: A CSV file with numerical.